воскресенье, 4 марта 2012 г.

Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics

ABSTRACT The establishment of specific molecular bonds between a cell and a facing surface is involved in many physiological and technological situations. Using micrometric magnetic particles, we have explored the formation of specific molecular bonds between the cell and surfaces bearing complementary ligands under passive conditions. Streptavidin-coated particles were targeted to the cell surface of a B-cell line through a specific biotinylated antibody against the CD19 receptor. Flow cytometry, optical microscopy, and micropipette experimental techniques have been used. Main findings have been that cell surface receptor density acted like a switch for particle capture with a threshold …

Комментариев нет:

Отправить комментарий